TABLE 3.
Intracellular metabolite profiles of a 35-deletion, minimal CCM prototrophic S. cerevisiae strain in aerobic bioreactor batch culturesa
| Pathway and metabolite | Amount of metabolite (μmol/g biomass [dry wt]−1) |
Fold difference | |
|---|---|---|---|
| CEN.PK113-7D (naive reference) | IMX2538 (minimal CCM) | ||
| Glycolysis | |||
| Glucose 6-phosphate | 4.13 ± 0.49 | 5.01 ± 0.38 | 1.2 |
| Fructose 6-phosphate | 0.38 ± 0.06 | 0.59 ± 0.08 | 1.5* |
| Fructose 1,6-bisphosphate | 17.53 ± 0.82 | 20.52 ± 0.66 | 1.2* |
| Glyceraldehyde 3-phosphate | 0.15 ± 0.01 | 0.19 ± 0.01 | 1.3* |
| Dihydroxyacetone phosphate | 0.79 ± 0.13 | 2.08 ± 0.66 | 2.6 |
| 3-Phosphoglycerate | 1.30 ± 0.12 | 2.52 ± 0.28 | 1.9* |
| 2-Phosphoglycerate | 0.16 ± 0.01 | 0.17 ± 0.01 | 1.0 |
| Phosphoenolpyruvate | 0.25 ± 0.01 | 0.12 ± 0.01 | 0.5* |
| Trehalose synthesis | |||
| Trehalose 6-phosphate | 9.34 ± 1.18 | 14.48 ± 1.67 | 1.6* |
| Trehalose | 0.20 ± 0.04 | 0.41 ± 0.09 | 2.1* |
| Pentose phosphate pathway | |||
| 6-Phosphoglucononate | 1.01 ± 0.02 | 1.11 ± 0.07 | 1.1 |
| Ribose 5-phosphate | 0.40 ± 0.02 | 0.51 ± 0.03 | 1.3* |
| Ribulose 5-phosphate | 0.20 ± 0.03 | 0.33 ± 0.06 | 1.6* |
| Xylulose 5-phosphate | 0.43 ± 0.07 | 0.72 ± 0.13 | 1.7 |
| Sedoheptulose 7-phosphate | 0.50 ± 0.06 | 0.54 ± 0.04 | 1.1 |
| Erythrose 4-phosphate | 0.004 ± 0.000 | 0.005 ± 0.000 | 1.3* |
| TCA cycle and glyoxylate cycle | |||
| Citrate | 4.56 ± 0.65 | 5.80 ± 0.98 | 1.3 |
| Isocitrate | 0.02 ± 0.00 | 0.03 ± 0.00 | 1.7* |
| α-Ketoglutarate | 0.29 ± 0.02 | 0.67 ± 0.24 | 2.3 |
| Succinate | 0.37 ± 0.03 | 0.37 ± 0.12 | 1.0 |
| Fumarate | 0.10 ± 0.02 | 0.18 ± 0.06 | 1.8 |
| Malate | 0.60 ± 0.08 | 1.03 ± 0.19 | 1.7* |
| Nucleotides and cofactors | |||
| Energy charge | 0.86 ± 0.00 | 0.88 ± 0.01 | 1.0 |
| AMP | 0.13 ± 0.01 | 0.17 ± 0.01 | 1.3* |
| ADP | 1.55 ± 0.08 | 1.43 ± 0.11 | 0.9 |
| ATP | 5.50 ± 0.16 | 5.58 ± 0.21 | 1.0 |
| UDP | 0.23 ± 0.01 | 0.22 ± 0.05 | 0.9 |
| UTP | 1.61 ± 0.06 | 1.57 ± 0.10 | 1.0 |
| GDP | 0.27 ± 0.02 | 0.21 ± 0.07 | 0.8 |
| GTP | 1.58 ± 0.06 | 1.49 ± 0.15 | 0.9 |
| CDP | 0.13 ± 0.02 | 0.15 ± 0.01 | 1.1 |
| CTP | 0.84 ± 0.02 | 0.82 ± 0.03 | 1.0 |
| NADH | 0.18 ± 0.02 | 0.15 ± 0.02 | 0.8 |
| NAD+ | 85.43 ± 1.90 | 91.82 ± 2.64 | 1.1* |
| NADP+ | 8.49 ± 0.67 | 6.87 ± 0.25 | 0.8* |
| Acetyl-CoA | 5.80 ± 0.18 | 5.38 ± 0.34 | 0.9 |
| FAD+ | 0.87 ± 0.12 | 0.54 ± 0.18 | 0.6 |
a
Intracellular metabolite contents were measured during the mid-exponential glucose phase of aerobic bioreactor batch cultures of S. cerevisiae CEN.PK113-7D (naive reference) and IMX2538 (minimal CCM) (see Table 2 for other physiological data). Data represent averages and standard deviations of data from analyses of three independent cultures for each strain. Fold differences that are statistically significant are indicated in bold with an asterisk (two-tailed t test, equal variances, P < 0.05).