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. 2022 Oct 18;11(20):3274. doi: 10.3390/cells11203274

Figure 2.

Figure 2

Regulation of TRAF adaptor proteins and pro-apoptotic MAP kinases following CD40 ligation by mCD40L in CRC cells. (a) TRAF1, TRAF3 and TRAF6 protein expression was detected by immunoblotting following co-culture of HCT116 and SW480-CD40 cells with 3T3-Neo [Control (C)] or 3T3-CD40L [mCD40L (mL)] at the indicated time points. Lysate from effector (3T3-CD40L) cell monocultures served as negative control (NC) to confirm the human-protein specificity of the antibodies. Equal loading for human epithelial cell lysate was confirmed by CK18 detection (as detailed in the Methods). (b) Phosphorylation of MKK4 (p-MKK4), p38 (p-p38) and JNK (p-JNK) was detected by immunoblotting following treatment of HCT116 and SW480-CD40 cells with mCD40L (mL) versus control (C) as in (a).