Skip to main content
. 2022 Aug 18;13(5):e01873-22. doi: 10.1128/mbio.01873-22

FIG 4.

FIG 4

r-protein quantities in the 70S ribosomes during the stationary phase. Cells were collected over the course of 14 days. 70S ribosomes were isolated and mixed in a 1:1 ratio with medium-heavy-labeled reference 70S ribosomes for r-protein quantification using LC-MS/MS. Proteins bL31A, bL31B, bL35, bL36A, and bL36B were not quantified due to insufficient numbers of unique peptides. Data sets from days 2, 6, and 12 are shown, and the complete set of all data sets is shown in Fig. S6 at http://dx.doi.org/10.23673/re-310. The relative quantities of 51 r-proteins are presented as the (L+H)/M ratios (L+H = sample; M = reference). 50S r-protein (L+H)/M ratios were normalized against the average (L+H)/M ratio of all 50S r-proteins. 30S r-protein (L+H)/M ratios were normalized against the average (L+H)/M ratio of all 30S r-proteins. The blue-shaded areas mark the ±10% range of (L+H)/M ratios. Values shown in the figure are the mean results from three independent biological experiments with standard deviations (n = 3; mean ± SD).