TABLE 2.
Antibodies used in this study
| Antibody | Dilution | Source |
|---|---|---|
| Primary antibodies | ||
| Mouse anti-FLAG | 1:5,000 | Sigma F3164 |
| Goat anti-ACE2 | 1:1,000 | R&D Systems AF933SP |
| Mouse anti-β-actin | 1:2,000 | Santa Cruz Biotechnology sc-47778 |
| Rabbit anti-ZMPSTE24 | 1:500 | Thermofisher 16965 |
| Rat anti-prelamin A (3C8) | 1:2,000 | Stephen Young, UCLA |
| Secondary antibodies | ||
| Donkey anti-mouse IRDye 680RD | 1:15,000 | LI-COR Biosciences |
| Goat anti-rat IRDye680RD | 1:15,000 | LI-COR Biosciences |
| Goat anti-rabbit IRDye 800CW | 1:15,000 | LI-COR Biosciences |
| Donkey anti-goat IRDye 800CW | 1:15,000 | LI-COR Biosciences |