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. 2022 Oct 20;14(20):5146. doi: 10.3390/cancers14205146

Figure 1.

Figure 1

Comparison of miRNA, mRNA and protein levels of selected epithelial to mesenchymal transition (EMT) and cell migration potential markers as measured by RT-PCR and Western blot analysis (Materials and methods). (A) mRNA and miRNA of EMT markers in HCT8 and HCT8_N-cad cells; (B) mRNA and miRNA of EMT markers in SW480 and SW480_N-cad cells; (C) mRNA and miRNA of EMT markers in 47B and 48 primocultures; (D) protein expression of selected EMT and cell migration potential markers in HCT8, SW480 and its _N-cad variants, 47B and 48B primocultures. Numbers indicate densitometric analysis results. B2M was used as a reference gene in RT-PCR. * p < 0.05 N-cadherin plasmid-treated vs. untreated cells, resp. 47B vs. 48B cells. α-tubulin was used as a reference protein in immunoblotting. * p < 0.05 plasmid N-cadherin-treated vs. untreated cells, resp. 47B vs. 48B; # p < 0.05 47B vs. 48B. Original western blots can be found in Supplementary File S1.