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RT-PCR and Western blot analysis of tumors 47B and 48B excised from athymic immunodeficient mice. (A) mRNA fold increase of EMT markers in 47B and 48B tumors; (B) protein fold increase of markers related with EMT; (C) drug resistance and cell motility markers in 47B and 48B tumors; B2M was used as a reference gene. ß-actin was used as a reference protein. * p < 0.05 means 47B vs. 48B tumors.
