FIG 2.

D256N and D270N substitutions restore IN-gRNA binding and accurate virion maturation for the HIV-1_{NL4-3 IN (R269A/K273A)} class II mutant virus. (A) Autoradiogram of IN-RNA adducts immunoprecipitated from virions bearing the indicated substitutions in IN. Immunoblots below show immunoprecipitated (IP) IN or CA protein in lysates prior to immunoprecipitation. Data shown are representative of three independent experiments. See also Fig. S2 for quantitative analysis of autoradiographs. (B) Examination of virion maturation in WT and IN mutant viruses by thin section electron microscopy (TEM). Data show quantification of virion morphologies across 100 particles for each sample and replicate experiment. Data show the average of two independent biological replicates, and error bars represent the SEM (***, P < 0.001; **, P < 0.01; ns = not significant, by one-way ANOVA multiple comparison test with Dunnett’s correction).