FIG 2.

Identification of essential genetic determinants for As^V resistance in V. cholerae. (Left) Experimental workflow for an arrayed transposon mutant screen to identify the genes required for As^V resistance in V. cholerae and the list of mutant strains identified. (Right) Experimental workflow for TIS screen in the presence and absence of 1 mM As^V. Volcano plot depicting the ratio of read counts mapped to individual genes in transposon libraries of (V. cholerae plated onto medium supplemented with As^V). Red dotted lines indicate arbitrary thresholds of a fold change of (FC) <0.1 and an inverse P value of >20. Genes in red show loci also identified in the arrayed screen (see Table S1 for an extended version).