Figure 3.

FISH interpretation criteria: (From Abbott Molecular, Vysis probes) (I) Count 50 tumor cells: If the number of positive cells was ≥50% (25), the patient was judged to have ALK rearrangements (On the right). If the number of positive cells was less than 10% (5), the patient was judged to have no ALK recombination (On the left); (II) If the positive cells were ≥10% (5) and <50% (25), another 50 tumor cells were counted, and then the 100 tumor cells were counted together. If the number of positive cells is ≥15%, the patient is judged to have ALK recombination. If the number of positive cells was less than 15%, the patient was judged to have no ALK recombination. Note: The positive cells were the cells with orange and green signal separation or orange signal alone.