Skip to main content
. 2022 Oct 11;11(20):3184. doi: 10.3390/cells11203184

Figure 3.

Figure 3

Fenofibrate alleviated cardiac fibrosis in CS-Dsg2−/− mice. (A) Masson staining of heart sections in WT, CS-Dsg2−/− mice, and CS-Dsg2−/− mice treated with fenofibrate (Dsg2−/−F). Collagen volume fraction in the hearts of WT, CS-Dsg2−/−, and Dsg2−/−F mice were assessed. (B) Representative Western blots from ventricles of WT, CS-Dsg2−/−, and Dsg2−/−F mice. DSG2, PPARα, pSTAT3, pSMAD3, pAKT, α-SMA, and Collagen I were detected using specific antibodies. STAT3, SMAD3, AKT, and GAPDH were used as loading controls. (C) Results of quantitative PCR analysis of PPARα, TGF-β, α-SMA, and Collagen I mRNA levels in mouse ventricles are expressed as fold change of control using β-actin as loading control. Results are expressed as mean values ± SEM. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control.