Optimization of rea ction conditions of enzymatic alcoholysis. Reaction conditions: (a) molar ratio of 1:40 (fungal oil/ethanol), 15% (relative to the mass of oil) Lipozyme TL IM, solvent volume of 1 mL, aw of 0.53, 35 °C, 8 h. (b) molar ratio of 1:40 (fungal oil/ethanol), 15% (relative to the mass of oil) Lipozyme TL IM, t-butanol as solvent, aw of 0.53, 35 °C, 8 h. (c) molar ratio of 1:40 (fungal oil/ethanol), 15% (relative to the mass of oil) Lipozyme TL IM, 3 mL t-butanol as solvent, 35 °C, 8 h. (d) molar ratio of 1:40 (fungal oil/ethanol), 15% (relative to the mass of oil) Lipozyme TL IM, 3 mL t-butanol as solvent, aw of 0.53. Different letters in the figure indicated significant differences at the p < 0.05 level.