Table 2.

The variability characteristics of single DNA molecular barcoding genes in Morchella.

Gene	Length (bp)	No. SNPs	% SNP	No. inDels	% No. Variable Sites	Intraspecific Distance (Mean)	Interspecific Distance (Mean)	NJ Rate (%) P-Distance	NJ Rate (%) K2P	PWG-Distance Rate (%) K2P
RPB1	420	94	22.38	2	22.85	0.00056	0.0673	33.33	35.56	53.33
RPB2	391	86	21.99	0	21.99	0.00057	0.0026	37.78	40.00	63.04
ITS	452	125	27.65	78	44.91	0.00062	0.1347	48.89	44.44	57.78
EF1-α	312	76	24.36	18	30.13	0.00040	0.077	46.67	42.22	53.33

Length, each single region’s aligned sequence length; No. SNPs, the number of SNPs; % SNP, percentage SNP calculated as the number of SNPs in relation to the longest sequence length; No. inDels, the number of insertions/deletions; % No. variable sites, the ratio of the sum of SNP and insertions/deletions sites to the longest sequence length; interspecific distance (mean), the barcoding gap between species; intraspecific distance (mean), the barcoding gap within species; rate (%), percentage of successful discrimination species calculated as the number of successful discrimination species in relation to the total species; NJ, tree-building method (neighbor-joining tree); PWG-distance, intraspecific and interspecific genetic distance approach; P-distance and K2P represent different model parameters.