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. 2022 Oct 15;13(10):1861. doi: 10.3390/genes13101861

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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miRNAs targeting orb2 as predicted by RNAhybrid and confirmation through dual luciferase reporter assay. (A) Potential miRNA target sites of miR-9c-3p, miR-125-3p, miR-210-5p, miR-276b-3p, and miR-3931-3p. Seed sequence of the miRNAs and their putative binding sites in the 3′UTR are indicated by red letters. mfe: indicate match free energy value. (B) Effects of miR-9c-3p, miR-125-3p, miR-210-5p, miR-276b-3p, and miR-3931-3p on luciferase activity. (C) The effects of combination of miR-125-3p/276b-3p on luciferase activity. Treatments were compared with their respective controls using ANOVA (Tukey-test, p < 0.05). *** indicates p < 0.001 and ns non-significant respectively.