. 2022 Oct 11;11(20):3158. doi: 10.3390/foods11203158

Table 6.

The effect of different antioxidants on the DHA, ALA, Tn-3, and Tn-6 of egg yolk during storage, expressed as mg/egg.

FA	Storage	Control	VE	CA	PF	L	SEM	p-Value ¹
DHA	0 d	65.10 ^bA	74.81 ^a	73.78 ^a	74.15 ^a	74.28 ^aA	0.71	<0.001
	7 d	65.10 ^bA	74.70 ^a	73.77 ^a	74.11 ^a	74.09 ^aA	0.71	<0.001
	14 d	64.70 ^bA	74.53 ^a	73.43 ^a	74.09 ^a	74.18 ^aA	0.73	<0.001
	21 d	64.19 ^bA	74.33 ^a	73.25 ^a	74.06 ^a	73.66 ^aA	0.76	<0.001
	28 d	60.83 ^bB	74.16 ^a	73.05 ^a	73.53 ^a	72.68 ^aAB	0.96	<0.001
	35 d	57.87 ^cC	73.96 ^a	72.92 ^a	73.01 ^a	70.70 ^bBC	1.15	<0.001
	42 d	52.69 ^cD	73.38 ^a	72.86 ^a	73.08 ^a	70.07 ^bC	1.53	<0.001
	p-value ²	<0.001	0.519	0.911	0.514	<0.001
	Linear ³	<0.001	0.033	0.171	0.043	<0.001
	Quadratic ⁴	<0.001	0.612	0.909	0.549	0.042
ALA	0 d	324.39 ^bA	332.41 ^a	331.46 ^a	333.12 ^a	331.76 ^a	0.75	<0.001
	7 d	324.25 ^bA	332.32 ^a	331.43 ^a	333.14 ^a	331.67 ^a	0.74	<0.001
	14 d	324.07 ^bA	332.23 ^a	331.28 ^a	333.07 ^a	331.48 ^a	0.75	<0.001
	21 d	322.52 ^bA	332.29 ^a	331.19 ^a	332.99 ^a	331.40 ^a	0.85	<0.001
	28 d	319.32 ^bB	331.71 ^a	330.82 ^a	332.64 ^a	331.41 ^a	1.02	<0.001
	35 d	315.75 ^bC	331.04 ^a	330.00 ^a	331.47 ^a	330.74 ^a	1.20	<0.001
	42 d	312.55 ^bD	329.74 ^a	329.56 ^a	331.00 ^a	329.41 ^a	1.36	<0.001
	p-value ²	<0.001	0.380	0.579	0.955	0.581
	Linear ³	<0.001	0.032	0.050	0.284	0.075
	Quadratic ⁴	<0.001	0.896	0.983	0.997	0.921
Total n-3	0 d	426.41 ^bA	436.49 ^a	435.34 ^a	437.55 ^a	435.80 ^aA	0.87	<0.001
	7 d	426.28 ^bA	436.28 ^a	435.15 ^a	437.37 ^a	435.70 ^aA	0.88	<0.001
	14 d	426.09 ^bA	436.25 ^a	435.12 ^a	437.15 ^a	435.44 ^aA	0.88	<0.001
	21 d	424.69 ^bAB	436.06 ^a	435.01 ^a	437.10 ^a	435.05 ^aA	0.97	<0.001
	28 d	423.63 ^bB	435.61 ^a	434.67 ^a	436.18 ^a	434.55 ^aA	0.96	<0.001
	35 d	419.24 ^bC	435.34 ^a	434.66 ^a	435.70 ^a	433.89 ^aA	1.24	<0.001
	42 d	409.94 ^cD	434.82 ^a	433.50 ^ab	434.65 ^a	431.15 ^bB	1.82	<0.001
	p-value ²	<0.001	0.934	0.823	0.845	0.005
	Linear ³	<0.001	0.206	0.152	0.133	<0.001
	Quadratic ⁴	<0.001	0.751	0.529	0.618	0.050
Total n-6	0 d	557.63 ^aA	549.73 ^c	551.57 ^bcA	553.95 ^bA	551.61 ^bc	0.64	<0.001
	7 d	557.59 ^aA	549.58 ^c	551.56 ^bcA	553.75 ^bA	551.57 ^bc	0.63	<0.001
	14 d	557.41 ^aA	549.39 ^c	551.40 ^bcA	553.55 ^bA	551.24 ^bc	0.64	<0.001
	21 d	556.57 ^aAB	549.42 ^c	551.37 ^bcA	552.92 ^bA	551.10 ^bc	0.61	0.001
	28 d	555.21 ^aBC	548.71 ^b	550.89 ^bAB	552.25 ^abAB	550.57 ^b	0.66	0.018
	35 d	554.53 ^aCD	547.56 ^b	547.93 ^bBC	550.92 ^abB	548.77 ^b	0.79	0.018
	42 d	552.97 ^D	546.47	546.95 ^C	547.92 ^C	547.74	0.82	0.073
	p-value ²	<0.001	0.468	0.018	<0.001	0.663
	Linear ³	<0.001	0.037	0.001	<0.001	0.074
	Quadratic ⁴	0.058	0.346	0.064	0.016	0.441

VE 0.02% = vitamin E; CA 0.24% = chlorogenic acid; PF 0.05% = polyphenol; L 0.03% = lutein; SEM = standard error of mean, n = 6. The small letters represent significant differences among treatments at one storage time (rows); a post-hoc Duncan test was used for means comparison (p-values ¹). The capitals letters represent significant differences among means for individual treatment from 0–42 d of storage (columns); a post-hoc Duncan test (p-values ²), linear (p-values ³), and quadratic (p-values ⁴) polynomial contrasts were applied for comparisons. Significance was set at p < 0.05 for all tests.