FIG. 1.

Amplification of DNA from standard plasmid dilutions and comparison of the amplification efficiencies of pOvHV-2 DNA (the average of four experiments is shown) and pSHgenom DNA (the average of three experiments is shown). The logarithmic value of the number of standard plasmid molecules per reaction mixture is indicated on the x axis, while the corresponding C_T values are given on the y axis. The calculated standard deviation values for each point, ranging from 0.01 to 0.5, are not indicated. For both regression curves, the coefficient of determination (R²) and the slope value (s) were calculated and are indicated. Diamonds, pOvHV-2; open circles, pSHgenom. Lin. regr., linear regression.