TABLE 1.
OvHV-2- and SHgenom-specific primers and probes used in fluorogenic assays
| Oligonucleotide | Sequence (5′→3′) | Length of fragment (bp) |
|---|---|---|
| Forward primer (oF-OvHV-2) | TGG TAG GAG CAG GCT ACC GT | 131 |
| Rerverse primer (oR-OvHV-2) | ATC ATG CTG ACC CCT TGC AG | |
| FAMa probe (oP-OvHV-2) | TCC ACG CCG TCC GCA CTG TAA GA | |
| Forward pimer (oF-SHgenom) | GTG CAA GGT GCC CTC GAC TA | 71 |
| Reverse primer (oR-SHgenom) | AGA AGC CTC GGG AGT GTG TCT | |
| VICb probe (oP-SHgenom) | TGA CCC TAG CAT GGC ACT CAG CCC |
a
FAM, 6-carboxyfluorescein.
b
VIC, commercial fluorescent dye with maximum intensity at 555 nm.