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Target sequences of BnFAD2 and BnFAE1 genes and vector construction. (a) Sequences of the target sites in BnFAD2 and BnFAE1 genes are shown. PAM sequences (NGG) are indicated in bold font. (b) Schematic of the genome editing vector. The vector contains a hygromycin phosphotransferase (HPT) gene as a selection marker and the 35S promoter (p35S) to drive CAS9.
