Figure 8.

Transcription profiles of MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70 genes in response to SA and MeJA phytohormones. A floating disc assay was performed in either SA (5 mM) or MeJA (100 µM) phytohormones. The leaf discs were incubated for 6 h and collected for total RNA extraction. Gene expression was evaluated by quantitative real-time PCR. The banana 25S gene was used as a reference gene for RT-qPCR analysis. The MaDLO1 and MaLOX1 genes were used as SA- and MeJA-responsive marker genes, respectively. The relative expression value was calculated by the 2^−ΔΔCT method [43]. The mean ± S.D. of three biological replicates is presented. Asterisks (*) indicate p ≤ 0.05 (t test).