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Protein spots selected for characterization by mass spectrometry (ESI-MS) after image analysis. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): 12.5% (w/v) and pH gradient 3–10. Muscle tissue samples (Longissimus thoracis) from feedlot-finished F1 Angus-Nellore bulls fed on diets without the inclusion of wet corn distiller grains (control).
