TABLE 3.

Biomolecular protocols with references and primer sequences used for arbovirus screening

Viruses	Protocols	Primer Fr	Primer R	Probe	Ref
WNV	RTa	WN10533-10552 AAGTTGAGTAGACGGTGCTG	WN10625-10606 AGACGGTTCTGAGGGCTTAC	WN10560-10579 CTCAACCCCAGGAGGACTGG	52
WNV/USUV	RT	WN-LCV-F1 GTGATCCATGTAAGCCCTCAGAA	WN-LCV-R1 GTCTGACATTGGGCTTTGAAGTTA	WN-LCV-S1 AGGACCCCACATGTT	53
				WN-LCV-S2 AGGACCCCACGTGCT
		USU-F ACGGCCCAAGCGAACAGAC	USU-R2 GGCTTGGGCCGCACCTAA	USU-S CGAACTGTTCGTGGAAGG
USUV	RT	USU-F -AAAAATGTACGCGGATGACACA	USU-R -TTTGGCCTCGTTGTCAAGATC	USU-P -CGGCTGGGACACCCGGATAACC	54
Pan-flavivirus	TSb	MAMD AACATGATGGGRAARAGRGARAA	cFD2 GTGTCCCAGCCGGCGGTGTCATCAGC		55
		FS 778 AARGGHAGYMCDGCHATHTGGT
Pan-orthobunyavirus	TS	BCS82C ATGACTGAGTTGGAGTTTCATGATGTCGC	BCS332V TGTTCCTGTTGCCAGGAAAAT		56
Pan-phlebovirus	TS	Phlebo forward 1 TTTGCTTATCAAGGATTTGATGC	Phlebo reverse TCAATCAGTCCAGCAAAGCTGGGATGCATCAT		57
		Phlebo forward 2 TTTGCTTATCAAGGATTTGACC

^aRT, real-time PCR.

^bTS, traditional PCR and sequencing.