Figure 1.

ONT Library Preparation Workflows. Generalized workflow for the four Oxford Nanopore Technology library preparation methods tested. Note that the 4-Primer method covers both the 4PSTD and 4PONT methods, the difference being the PCR cycling parameters. The bar chart displays total time from extracted gDNA to loading onto an ONT flow cell and broken down by the two primary processes: “Amplicon Generation” and “Sequencing Library Generation”: Amplicon Generation includes all steps from gDNA to PCR product, and Sequencing Library Generation includes all steps from PCR product to loading an ONT flow cell.