Table 3.
Minimum sequencing time required for TP calls. Minimum sequencing time required for TP calls for (A) FL type flow cell and (B) R9 type flow cell. TP was defined as >9 reads aligning to the associated amplicon reference in all three replicates per sample.
| Minutes until All TP Amplicons > 9 Reads | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Device | Flongle Flow Cell | R9 Flow Cell | |||||||
| Method | 4PONT | 4PSTD | LIGTN | NATBC | 4PONT | 4PSTD | LIGTN | NATBC | |
| Organism ID | BANT708 | >60 | >60 | >60 | 10 | >60 | >60 | 15 | 5 |
| BCER248 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | |
| BRUC105 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | |
| BRUC106 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | |
| BURK164 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | 5 | |
| BURK197 | >60 | >60 | >60 | 45 | >60 | >60 | >60 | 10 | |
| FRAN239 | >60 | >60 | 25 | 5 | >60 | >60 | 5 | 5 | |
| FRAN240 | >60 | >60 | 35 | 5 | >60 | >60 | 10 | 5 | |
| FRAN241 | >60 | >60 | 50 | 5 | >60 | >60 | 10 | 5 | |
| VACCIN | >60 | >60 | >60 | >60 | >60 | >60 | >60 | 5 | |
| YERS113 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | 5 | |
| YERS114 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | 10 | |
| Min | >60 | >60 | 25 | 10 | >60 | >60 | 5 | 5 | |
| Max | >60 | >60 | >60 | >60 | >60 | >60 | >60 | >60 | |