TABLE 2.
Enzyme genes upregulated on DAP but not on Lys in Thalassospira sp. strain A40-3a
| Gene (protein) | DAP |
Lysine |
||
|---|---|---|---|---|
| Fold changeb | P valuec | Fold changeb | P valuec | |
| carB (carbamoyl-phosphate synthase) | 8.66 | 1.66E−08 | −1.19 | 3.29E−15 |
| metB (O-succinylhomoserine sulfhydrylase) | 7.78 | 7.39E−15 | −1.97 | 2.52E−52 |
| thrA (aspartokinase) | 6.37 | 2.65E−265 | −0.61 | 5.02E−04 |
| lysA (diaminopimelate decarboxylase) | 6.11 | 1.01E−092 | 0.75 | 2.08E−07 |
| aroA (cytidylate kinase) | 5.30 | 1.13E−07 | −0.84 | 4.31E−06 |
| aro (B3-dehydroquinate synthase) | 5.13 | 2.86E−07 | 0.62 | 3.18E−06 |
| hisA (imidazole-4-carboxamide isomerase) | 5.04 | 4.68E−07 | 0.82 | 2.91E−05 |
| pyc (pyruvate carboxylase) | 2.95 | 2.35E−51 | −3.93 | 9.44E−181 |
| fumA (fumarate hydratase class I) | 2.56 | 4.00E−42 | −3.88 | 6.62E−138 |
| glyA (serine hydroxymethyltransferase) | 2.31 | 1.39E−27 | 0.36 | 7.17E−03 |
| HPD (hemolysin) | 2.19 | 2.20E−03 | −5.98 | 4.27E−190 |
| glcD (glycolate oxidase subunit) | 2.09 | 1.78E−27 | 0.66 | 8.52E−06 |
The resting cells of the strain were cultured with DAP or Lys as the sole N source to the middle of the logarithmic growth phase, and then cells were collected as the DAP sample and the Lys sample. The resting cells in sea salt solution were taken as the 0-h sample.
Calculated by comparing the transcription level of the gene in the DAP or Lys sample to that in the 0-h sample.
Calculated by Fisher’s exact test with FDR correction.