FIG 2.

MtrAB TCS is required for septation in Dietzia sp. DQ12-45-1b at pH 10. (A) Fluorescence microscopy of septation in the Dietzia sp. DQ12-45-1b wild-type strain, the ΔmtrAB mutant, and complementary strain cells in the exponential growth phase at pH 8 and pH 10. The cell membrane and DNA were marked using FM4-64 (red) and DAPI (blue), respectively. (B) Stacked columns of the percentages of cells containing a different number of septa from A were analyzed at pH 8 and pH 10 (~200 cells/sample).