FIG. 2.
Analysis of whole-cell lysates of strains positive for the AIDA system by immunoblot assays. Whole-cell lysates were separated on SDS–10% polyacrylamide gels, and proteins were detected by Western blot analysis with specific antibodies raised against AIDA-I and AIDAC. (A) Samples from PCR of strains obtained from animals diagnosed with ED or PWD. (B) Samples from PCR of strain isolated from animals not diagnosed with ED or PWD. precursor, proteins resembling the uncleaved pre-pro-protein of the AIDA system; AIDA-I, proteins resembling the adhesin of the AIDA system; AIDAC, proteins resembling the autotransporter of the AIDA system.