TABLE 1.

Primer sequences used for amplification of fragments derived from AIDA, F18, and Stx2e

Target region	Primer	Oligonucleotide sequence (5′→3′)	Product size (bp)	Reference
orfAa	UN19	CTGGGTGACATTATTGCTTGG	370	This study
	UN20	TTTGCTTGTGCGGTAGACTG
orfBb	UN21	TGCAAACATTAAGGGCTCG	450	This study
	UN22	CCGGAAACATTGACCATACC
orfBc	UN23	CAGTTTATCAATCAGCTCGGG	543	This study
	UN24	CCACCGTTCCGTTATCCTC
fedAd	fedA1	GTGAAAAGACTAGTGTTTATTTC	230	10
	fedA2	CTTGTAAGTAACCGCGTAAGC
stx2ee	Vte-a	CCTTAACTAAAAGGAATATA	510	15
	Vte-b	CTGGTGGTGTATGATTAATA

^aGiving rise to a fragment from the coding region for ORFA. 

^bGiving rise to a fragment from the coding region for AIDA-I (nucleotides 1014 to 1556). 

^cGiving rise to a fragment from the coding region for AIDA^C (nucleotides 3131 to 3580). 

^dGiving rise to a fragment from the coding region for fedA, the major subunit of F18. 

^eGiving rise to a fragment from the coding region for Stx2e.