Skip to main content
. 2001 Jan;8(1):150–160. doi: 10.1128/CDLI.8.1.150-160.2001

TABLE 1.

Characteristics of RPLs

Library No. of clonesa Vector Displayed peptideb Conformational constraint Copy no.c Host coat proteind
f3-6mer 2 × 108 fUSE5 ADGAX6 None 5 pIII
f3-15mer 2.5 × 108 fUSE5 ADGAX15 None 5 pIII
f88-15mer 2 × 109 f88-4 X15 None ∼150 pVIII
f88-Cys0 5.6 × 108 f88-4 AX5CCX5 Disulfide ∼150 pVIII
f88-Cys1 2.8 × 109 f88-4 AX5CXCX5 Disulfide ∼150 pVIII
f88-Cys2 5.5 × 107 f88-4 AX5CX2CX5 Disulfide ∼150 pVIII
f88-Cys3 5.2 × 107 f88-4 AX5CX3CX4 Disulfide ∼150 pVIII
f88-Cys4 1.7 × 108 f88-4 AX4CX4CX5 Disulfide ∼150 pVIII
f88-Cys5 5.9 × 108 f88-4 AX4CX5CX4 Disulfide ∼150 pVIII
f88-Cys6 2.7 × 108 f88-4 AX4CX6CX4 Disulfide ∼150 pVIII
f88-LX6 1010 f88-4 XCX6CX Disulfide ∼150 pVIII
f8-8mer 2 × 109 f8-1 AX8 Close packing 3,900 pVIII
a

For all libraries but f3-6mer, the number of primary clones is also the number of different displayed peptides. 

b

Each sequence starts at the N terminus of the coat protein (just after the signal peptidase cleavage site) and extends through the last randomized amino acid. X, randomized amino acids; A, C, D, or G, invariant amino acids. 

c

Estimated number of copies of the peptide displayed per virion. 

d

Host protein to which the displayed peptide is genetically fused: either pIII (5 subunits in a ring at one tip of the virion) or pVIII (3,900 subunits arranged to form the tubular covering of the virion).