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. 2022 Sep 8;10(5):e02934-22. doi: 10.1128/spectrum.02934-22

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Copyright © 2022 Cui et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — Generation and characterization of artificially recombinant PRRSVs. (A) Schematic diagram for constructing recombinant plasmids. (B) Virus detection by immunofluorescence. Recombinant and parental plasmids (4 μg) were transfected into Marc-145 cells. At 5 days posttransfection, the transfected cells were fixed and stained with monoclonal antibody 3F7 against PRRSV, followed by incubation with Alexa Fluor 488-conjugated goat anti-mouse IgG. Scale bar, 400 μm. The growth kinetics of the recombinant (rHeB108-HuN4NSP9 and rHeB108-HuN4NSP9p) and parental viruses in PAMs (C and D) and Marc-145 cells (E and F) are represented. Each data point represents the mean value of three replicates with the SD. *, Significant difference between rHeB108 and rHeB108-HuN4NSP9 (*, P < 0.05; **, P < 0.01; ***, P < 0.001); #, significant difference between rHeB108 and rHeB108-HuN4NSP9p (#, P < 0.05; ##, P < 0.01; ###, P < 0.001); δ, significant difference between rHuN4 and rHeB108-HuN4NSP9 (δ, P < 0.05; δδ, P < 0.01; δδδ, P < 0.001); ψ, significant difference between rHuN4 and rHeB108-HuN4NSP9p (ψ, P < 0.05; ψψ, P < 0.01; ψψψ, P < 0.001).