Figure 2.

Decorin requires the Met receptor kinase to trigger depolarization in carcinoma cells. A–H: gallery of live cell images of 231 cells treated in various combinations of decorin (100 nM), SU11274 (1 µM), or AG1478 (1 µM) and stained with JC-10. I: quantification of the JC-10 signal from A–H. J–Q: identical experiment as in A–H performed in HeLa cells. R: quantification of the JC-10 signal from J–Q. S and T: identical experiments as performed above with combinations of decorin and TKi but stained with TMRE in 231 (S) or HeLa cells (T). DMSO served as vehicle to account for SU11274 and AG1478 for all experiments. All data presented are reflective of several (n = 4) independent biological replicates. Data presented are expressed as means ± SE. Statistics calculated via one-way ANOVA (***P < 0.001).