Figure 3.

The Met receptor is required for decorin-dependent tumor cell depolarization. Representative immunoblots confirming the efficacy of Met depletion in 231 (A) or HeLa (B) cells via siRNA with densitometry values below the loading control. Amounts of siRNA for siScr and siMet were kept constant throughout (100 pM). C: gallery of live cell images depicting 231 or HeLa cells following transient knockdown of the Met receptor, challenged with decorin (6 h, 100 nM), and then stained with either JC-1 (top) or JC-10 (bottom). D and E: quantification of the JC-1 signal in 231 (D) or HeLa (E) from data in C. F and G: quantification of the JC-10 signal in 231 (F) or HeLa (G) from data in C. H and I: identical Met knockdown experiment as in C, but stained with TMRE in 231 (H) or HeLa (I). J and K: quantification of the resultant TMRE signal in 231 (J) or HeLa (K) after being stimulated with a combination, as noted, of decorin (100 nM) or HGF (50 ng/mL) for 6 h. L and M: Met rescue experiment done in 231 (L) or HeLa (M) and stained with TMRE as per the conditions listed. Vehicle (pcDNA3.1) and active plasmid (myc-Met) were used at equimolar amounts (4 µg). For immunoblots A and B, α-tubulin served as the loading control. PBS served as vehicle for all experiments. All data presented are reflective of several (n = 4, unless otherwise noted) independent biological replicates. Data are expressed as means ± SE. Statistics calculated via one-way ANOVA (***P < 0.001).