Figure 6.

Mitostatin and mitofusin 2 are in the same pathway and required for depolarization. A: representative immunoblots confirming the efficacy of mitostatin and/or mitofusin 2 depletion via siRNA in 231 cells in combination with decorin (6 h, 100 nM) as noted. B: gallery of live cell images depicting JC-10 staining in 231 cells under a variety of experimental conditions as labeled. C: quantification of the JC-10 signal in B. D: representative immunoblots confirming the efficacy of mitostatin and/or mitofusin 2 depletion via siRNA in HeLa cells in combination with decorin (6 h, 100 nM) as noted. E: gallery of live cell images depicting JC-10 staining in HeLa cells under a variety of experimental conditions as indicated. F: quantification of the JC-10 signal in E. For immunoblots (A and D), GAPDH served as the loading control. PBS served as vehicle for all experiments. All data presented are expressed as n = 4 independent biological replicates. Data are expressed as means ± SE. Statistics calculated via one-way ANOVA (***P < 0.001).