Figure 7.

Decorin relies on the canonical mPTP to trigger tumor cell depolarization. A–I: gallery of live cell images depicting 231 cells treated in a variety of combinations with decorin (100 nM), carbonylcyanide 4-triflouromethoxy phenylhydrazone (FCCP; 1 µM), cyclosporin A (5 µM), or NIM811 (5 µM) and stained with JC-10. J: quantification of the JC-10 signal as in (A–I). K–S: gallery of live cell images depicting an identical experiment as in (A–I) carried out in HeLa cells. T: quantification of the JC-10 signal as in K–S. U and V: quantification of the resultant tetramethyl rhodamine ethyl ester (TMRE) signal under identical conditions (e.g., A–I; K–S) performed in 231 (U) or HeLa (V). Dimethyl sulfoxide (DMSO) served as vehicle for all experiments to account for cyclosporin A and NIM811. All data presented are expressed as n = 4 independent biological replicates. Data are expressed as means ± SE. Statistics calculated via one-way ANOVA (***P < 0.001).