Figure 3.

Effect of exosomes from the three corneal cell types on hCECs proliferation. hCECs were incubated with 800–1000 μg exosomes isolated from medium of hCFs, hCECs and hCEnCs conditioned for 48 h. HBS was used as control (CTRL). Cells were fixed and Ki-67 was labeled by indirect immunofluorescence. Panel (A) shows representative images of Ki-67 expression in hCECs. Nuclei were counterstained with Hoechst 33258 reagent (blue). Scale bars: 100 μm. (B) The number of Ki-67 positive cells was calculated and plotted on graph. Between four and eight photos per condition were used for the counts. The data is expressed as the mean ± SEM from three independent experiments on two different populations. ** p < 0.01, **** p < 0.001, ns: not significant.