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. 2022 Sep 21;10(5):e02509-22. doi: 10.1128/spectrum.02509-22

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Copyright © 2022 Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Amoxapine suppresses intracellular Mycobacterium tuberculosis (Mtb) survival in macrophages. (A to C) Murine bone marrow-derived macrophages (BMDMs) were infected with Mtb H37Rv at an MOI of 10 for 4 h and treated with Amoxapine at indicated concentrations, or they were uninfected and treated with Amoxapine for 3 days. (A and B) LC3B-II and actin levels in murine BMDMs were determined by Western blots and quantified by Image J. (C) Intracellular survival of Mtb H37Rv was determined by CFU enumeration. (D and E) THP1 cells were infected with Mtb H37Rv at an MOI of 10 for 4 h and then treated with Amoxapine at indicated concentrations or uninfected and treated with Amoxapine for 3 days. LC3B-II levels were determined by Western blots and quantified by Image J. (F) Intracellular survival of Mtb H37Rv in THP1 cells was determined by CFU enumeration. Data represent mean ± standard deviations for three independent experiments. One-way ANOVA with Dunnett’s multiple-comparison test or Student’s t test was used to compare drug-treated groups to the control group. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.