TABLE 2.
PCR primers used in this study
| Object species | Target genes | Primer names | Sequences (5′–3′) | Round |
|---|---|---|---|---|
| Spotted fever group Rickettsia | rrs | S1 | TGATCCTGGCTCAGAACGAAC | 1st |
| S2 | TAAGGAGGTAATCCAGCCGC | 1st | ||
| S3 | AACACATGCAAGTCGRACGG | 2nd | ||
| S4 | GGCTGCCTCTTGCGTTAGCT | 2nd | ||
| gltA | CS2d | ATGACCAATGAAAATAATAAT | 1st | |
| CSEndr | CTTATACTCTCTATGTACA | 1st | ||
| RpCS.877p | GGGGACCTGCTCACGGCGG | 2nd | ||
| RpCS.1258n | ATTGCAAAAAGTACAGTGAACA | 2nd | ||
| Anaplasma spp. and Ehrlichia spp. | rrs | Eh-out1 | TTGAGAGTTTGATCCTGGCTCAGAACG | 1st |
| Eh-out2 | CACCTCTACACTAGGAATTCCGCTATC | 1st | ||
| Eh-gs1 | GTAATAACTGTATAATCCCTG | 2nd | ||
| Eh-gs2 | GTACCGTCATTATCTTCCCTA | 2nd | ||
| Anaplasma spp. | rrs | An16s1 | GTCACTGACCCAACCTTAAATGGCTGC | 1st |
| An16s2 | ATCCTGGCTCAGAACGAACGCTGG | 1st | ||
| An16s3 | GCGCCCTTCCGTTAAGAAGGATCTA | 2nd | ||
| An16s4 | AGCTTAACACATGCAAGTCGAACGGA | 2nd | ||
| Ehrlichia spp. | gltA | e-gltAwf | TTCTCAGGAATACATGCCACC | 1st |
| e-gltAwr | ACCATTGAGCAGACCAGCCA | 1st | ||
| e-gltAnf | AATTGCAGGGATAGTGGCAA | 2nd | ||
| e-gltAnr | CTGTGGCCAAAACCCATCAA | 2nd | ||
| Coxiella spp. | rrs | Cox16SF1 | CGTAGGAATCTACCTTRTAGWGG | 1st |
| Cox16SR2 | GCCTACCCGCTTCTGGTACAATT | 1st | ||
| Cox16SF1 | CGTAGGAATCTACCTTRTAGWGG | 2nd | ||
| Cox16SR1 | ACTYYCCAACAGCTAGTTCTCA | 2nd | ||
| Tick | Large subunit ribosomal RNA gene | TickHF | GGTATTTTGACTATACAAAGGTATTG | 1st |
| TickHR | TTATTACGCTGTTATCCCTAGAGTATT | 1st |