Figure 3.

ACP induction promotes pancreas specific activation of PI3K/AKT/mTOR/CREB signaling. A: Western blot analysis of C57BL/6 mice pancreata demonstrating highest activation of pAKT after ACP induction as compared with either control, alcohol (A), or CP-induced mice. B and C: representative image of phosphokinase array membranes (left) and its corresponding quantification (right) on pancreatic tissue lysates harvested from control, and ACP-induction with continuation of alcohol for 3 days during recovery period in C57BL/6 mice. D and E: Western blot analysis and its quantification to validate increased phosphorylated levels of pAKT, p70S6K, and CREB in mice pancreata obtained after ACP induction with continuation of alcohol for 3 days as compared with controls, n = 4 mice/group. F: representative photomicrographs of IHC based imaging analysis demonstrating acinar cell specific expression of pAKT and p70S6K in the pancreatic tissue sections of control and ACP-induced mice. Individual data points obtained from each mouse with means ± SD are reported and compared by two-tailed unpaired t test. *P < 0.05. ACP, alcoholic chronic pancreatitis; CP, chronic pancreatitis; IHC, immunohistochemistry; PI3K, phosphatidylinositol 3 kinase.