Figure 5.

Uro A treatment attenuates ACP severity in C57BL/6 mice even with continuous alcohol uptake by ameliorating acinar cell damage and fibroinflammatory milieu. A: representative photomicrographs of pancreas histology in control, ACP, and ACP+Uro A treated (both after 3 days and 2 wk of recovery period with continuation of alcohol diet) C57BL/6 mice depicted through H&E based analysis, collagen levels (Sirius red and trichrome blue), and PSC activation (αSMA immunoreactivity), (left). Scale bars, 50 μm. Image J based quantitative assessment of collagen levels and αSMA expression in the pancreas (right). B: immunohistochemical staining and quantification of F4/80 expression in the pancreatic tissue sections of control, ACP, and ACP+Uro A treated with 3 and 14 days of recovery period in C57BL/6 mice. Scale bars, 50 μm. C: ELISA based estimation of serum cytokines, IL-1β, IL-6, and SDF-1, from C57BL/6 mice treated with ACP and ACP+Uro A with 3 and 14 days of recovery period. Individual data points with means ± SD are shown and compared by one-way ANOVA for multiple comparisons or by two-tailed unpaired t test for two group comparison. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. n = 5 mice/group. ACP, alcoholic chronic pancreatitis; CP, chronic pancreatitis; H&E, hematoxylin and eosin; ns, nonsignificant; Uro A, urolithin A.