Figure 3.

AZD5634 inhibits I_sc in F508del-homozygous HBEC cultures. A: representative tracing using modified Ussing chambers with sequential addition of increasing concentrations of AZD5634, benzamil, or vehicle, followed by the addition of benzamil, the establishment of a chloride gradient, and then the addition of forskolin and CFTRinh-172. B: quantitation of cumulative drug effect on ion transport with summary data showing change in cumulative I_sc (C) through maximum drug treatment concentration (10 µM). D: comparisons of change in I_sc elicited by AZD5634, benzamil, or vehicle at each concentration tested. Summary data showing change in I_sc following acute addition of benzamil (E). n = 8 monolayers for AZD5634, n = 3 monolayers each for benzamil and vehicle. Means ± SE, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. HBEC, human bronchial epithelial cell.