Figure 4.

AZD5634 improves airway hydration and mucus transport in F508del-homozygous HBEC cultures. A–K: representative µOCT images depicting the effect of vehicle control (A), AZD5634 (C), lumacaftor/ivacaftor (luma/iva; E), and AZD5634 in combination with luma/iva (G) on airway surface liquid (ASL) depth at 6 h. (m) mucus, (ep) epithelium, blue arrow = cilia, red bar = ASL depth. B and D–F: images were resliced for calculation of mucociliary transport (MCT) rate, which was measured by projecting a cross-sectional line through the mucus through time. The slope of the particle trajectories indicated velocity. Change from baseline in ASL depth (I), MCT rate (J), and CBF (K) was assessed at 6 and 24 h post treatment. n = 17–18 monolayers per condition from a total of three different CF donors. Means ± SE, **P < 0.01, ***P < 0.001, and ****P < 0.0001. CBF, ciliary beat frequency; HBEC, human bronchial epithelial cell.