TABLE 2.
The results of 21 tet(X4)/tet(X6)-carrying strains by long-read Nanopore sequencing
| Strain | Location of tet(X) | Length (bp) | MGEa type | Resistance genes on tet(X)-harboring MGE | Conjugation frequency | Accession no. |
|---|---|---|---|---|---|---|
| JZ30 | pJZ30-tet(X4) | 185,363 | IncA/C2 | tet(X4), sul1, aac(3)-VIa, aadA1, erm(42), blaCMY-2, qacE, floR | 10−3 | ON390809 |
| JZ35 | ICEPmiChn-JZ35 | 142,200 | ICE | tet(X6), aph(3′)-VIa, aadA2b, aadA2, aph(6)-Id, aph(3′')-Ib, sul1, sul2, dfrA19, floR, qacE | 10−5 | ON390821 |
| JZ49 | pJZ49-tet(X4) | 197,640 | IncA/C2 | tet(X4), aac(3)-VIa, aadA1, erm(42), blaCMY-2, sul1, floR, qacE | 10−5 | ON390810 |
| JZ2 | pJZ2-tet(X4) | 31,272 | IncX1 | tet(X4), aadA2, floR, lnu(F), tet(A) | 10−5 | ON390804 |
| JZ18 | pJZ18-tet(X4) | 57,105 | IncX1 | tet(X4), aadA2, blaSHV-12, floR, lnu(F), tet(A) | 10−2 | ON390805 |
| JZ19 | pJZ19-tet(X4) | 57,105 | IncX1 | tet(X4), aadA2, blaSHV-12, floR, lnu(F), tet(A) | ON390806 | |
| JZ50 | pJZ50-tet(X4) | 31,250 | IncX1 | tet(X4), aadA2, floR, lnu(F), tet(A) | 10−3 | ON390811 |
| JZ21 | pJZ21-tet(X4) | 73,084 | IncX1 | tet(X4), aph(6)-Id, blaTEM-1B, sul3, dfrA14, floR, mef(B), qnrS1, formA, tet(A) | ON390807 | |
| JZ27 | pJZ27-tet(X4) | 159,632 | IncFIB, IncFIC | tet(X4), aph(3′)-Ia, aadA1, aadA2b, erm(42), sul3, floR, lnu(F), qnrS1, tet(A) | 10−4 | ON390808 |
| XY36 | pXY36-tet(X4) | 190,661 | IncFIA, IncHI1A, IncHI1B | tet(X4), aadA22, blaTEM-1B, qnrS1, floR, lnu(G) | ON390820 | |
| XY10 | pXY10-tet(X4) | 31,272 | IncX1 | tet(X4), aadA2, floR, lnu(F), tet(A) | 10−6 | ON390818 |
| XY14 | pXY14-tet(X4) | 31,272 | IncX1 | tet(X4), aadA2, floR, lnu(F), tet(A) | 10−5 | ON390819 |
| DM13 | pDM13-tet(X4) | 241,960 | IncFIA, IncHI1A, IncHI1B, IncX1 | tet(X4), aph(3′)-Ia, aadA22, blaTEM-1B, blaTEM-176, dfrA14, floR, lnu(G), qnrS1, tet(A) | 10−7 | ON390802 |
| TQ3 | pTQ3-tet(X4) | 31,271 | IncX1 | tet(X4), aadA2, floR, lnu(F), tet(A) | ON390813 | |
| TQ6 | pTQ6-tet(X4) | 201,404 | IncFIA, IncHI1A, IncHI1B | tet(X4), blaTEM-1B, floR, lnu(G), aadA22, qnrS1 | 10−4 | ON390814 |
| TQ2 | pTQ2-tet(X4) | 198,381 | Col, IncFIA, IncHI1A, IncHI1B | tet(X4), blaTEM-1B, floR, lnu(G), aadA22, qnrS1 | ON390812 | |
| TQ17 | pTQ17-tet(X4) | 90,608 | IncFIA, IncFIB, IncX1 | tet(X4), aadA2, blaTEM-1A, floR, lnu(F), qnrS1, tet(A) | ON390815 | |
| TQ30 | pTQ30-tet(X4) | 225,359 | IncX3, IncFIA, IncHI1A, IncHI1B | tet(X4), blaTEM-1B, floR, lnu(G), aadA22, qnrS1 | 10−3 | ON390817 |
| TQ12 | ICEPmiChn-TQ12 | 147,546 | ICE | tet(X6), aadA2, aph(3′)-Ia, aph(3′')-Ib, aph(6)-Id, sul2, floR, tet(A) | 10−4 | ON390822 |
| TQ28 | pTQ28-tet(X4) | 187,191 | IncFIA, IncHI1A, IncHI1B | tet(X4), blaTEM-1B, floR, aadA1, qnrS1, lnu(G) | 10−6 | ON390816 |
| DW28 | pDW28-tet(X4) | 46,856 | IncX1 | tet(X4), floR, lnu(F), aadA2, tet(A) | 10−5 | ON390803 |
a
MGE, mobile genetic element.