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Parameters and acceptance criteria for validation
| Parameter | Acceptance criterion |
|---|---|
| Limit of detection (LoD) | Lowest concn of com1 target that is detected in ≥95% of replicates |
| Limit of quantitation (LoQ) | Lowest concn of com1 target that can be quantified with a coefficient of variation (CV) of <25% (44) |
| Overall extraction efficiency | Mean of extraction efficiency at each concn above the LoQ |
