Skip to main content
. 2022 Oct 14;23(20):12303. doi: 10.3390/ijms232012303

Figure 5.

Figure 5

NDRG1 downregulated the NF-κΒ/ZEB1 pathway in endometrial epithelial cells. (A) Effect of NDRG1 depletion on p65 mRNA expression in RL95-2 cells. (B) Effect of NDRG1 depletion on ZEB1 mRNA expression in RL95-2 cells. (C) Analysis of p65 and ZEB1 protein after NDRG1 depletion in RL95-2 cells. (D) Effect of NDRG1 overexpression on p65 mRNA expression in AN3-CA cells. (E) Effect of NDRG1 overexpression on ZEB1 mRNA expression in AN3-CA cells. (F) Analysis of p65 and ZEB1 protein after NDRG1 overexpression in AN3-CA cells. (G,H) Immunofluorescence images of p65 protein expression in NDRG1-depleted or -overexpressing cells. (I,J) Immunofluorescence images of ZEB1 protein expression in NDRG1-depleted or -overexpressing cells. (K) Quantification of immunofluorescence images using image J. All images are shown at 400× magnification. Scale bars indicate 20 μm. Nuclei were stained with DAPI. The data shown represent the mean ± SEM from three independent experiments (n = 3). * p < 0.05; ** p < 0.01.