Figure 2.

Obese db/db mice have depressed hepatic Per2. A: hepatic PER2 reporter activity in control and db/db mice at time points indicated. Reporter activity was assessed using Renilla luciferase assay in 50-mg samples using luminometer. Hepatic Per2 (B) and Dbp (C) mRNA expression in control and db/db mice at time points indicated; 18 s was used as internal control. D: renal PER2 reporter activity in control and db/db mice at time points indicated. Reporter activity was assessed using Renilla luciferase assay in 50-mg samples using luminometer. Shaded areas represent time of subjective night. Data shown as means ± SE; n = 3 or 4 per group for each time point. *Significantly different (P < 0.05) using two-factor ANOVA.