Figure 3.

BrTyr released from a pooled urine after incubation with or without β-glucuronidase. A: urine BrTyr released after incubation with 150 units β-glucuronidase/mL at RT, 37°C, and 45°C for different time points. Urine was buffered with 0.3 M ammonium acetate buffer, pH 5.0. The released BrTyr was mixed with internal standard mix followed by solid phase extraction as described in methods. B: without enzymatic incubation, urine BrTyr released after exposure to RT, 37°C, and 45°C for different time points. BrTyr, 3-bromotyrosine; RT, room temperature.