FIG 1.

FISH analysis of liver cancer. Formalin-fixed paraffin-embedded (FFPE) liver cancer sections were deparaffinized, rehydrated, and probed with Cy3-labeled probes EUB338 (5′-GCTGCCTCCCGTAGGAGT-3′) (red) or Cy5-labeled nonspecific complement probe (5′-CGACGGAGGGCATCCTCA-3′) (pink). Both probes have been recently used to analyze the intratumoral microbiota of human cancer tissues (13). The nuclei were counterstained with diamidino-phenyl-indole (DAPI). A. Single-mode image of the staining. The yellow box indicates the region enlarged in the low right. B. DAPI staining of the nuclei. C. EUB338 staining. D. Negative staining. E. The merged image of different staining. The yellow arrows indicate clustered RBCs in the congestion area. The arrowheads indicate RBCs outside the congestion area. F. Hematoxylin-eosin staining of a sequential tissue section. The region corresponding to the FISH image was shown. RBC, red blood cell. HC, hepatocyte. CV, central vein.