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The expression of pmrCAB mRNA in representative A. baumannii strains of pmrAB mutants (CM022S [pmrA], CM012S [pmrB], and wild-type strain ATCC 19606) was analyzed by qRT-PCR. The strains were grown in LB broth at 37°C with shaking until they reached an OD600 of 0.75. Total RNA was extracted and subjected to qRT-PCR by the intercalator method (TB Green). Data are means and SD; n = 3 per group, compared by one-way ANOVA. n.s., not significant; ***, P < 0.001; **, P < 0.01; *, P < 0.05.
