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. 2022 Oct 11;23(20):12090. doi: 10.3390/ijms232012090

Figure 9.

Figure 9

IL11 inhibited the expression of IDO1. (A) mRNA expression estimated by RT-PCR for IDO1 after supplementing rmIL11 in NIH3T3 and L929 cells. (B) Western blot analysis and densitometric analysis of IDO1 protein levels after supplementing rmIL11 in NIH3T3 and L929 cells; GAPDH was used as the loading control. (C) Representative immunocytochemistry staining of IDO1 and cell nuclei (Hoechst) after supplementing rmIL11 in NIH3T3 and L929 cells. (D) IDO1 content estimated by ELISA from treated cell lysate. (E) The relative KYN level was detected by Ehrlich assay after inhibiting IL11 in NIH3T3 and L929 cells. OGD—oxygen–glucose deprivation; CON—control. The results are presented as the means ± SDs, and Student’s t-test was used to determine the p-value. * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with CON group in NIH3T3 cells; # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with CON group in L929 cells.