FIG 4.
Expression levels of PepO and phosphorylated CovR in the wild-type strains, the rocA mutants, the rocA trans-complementary strain, and the emm3-type clinical isolates. (A) The expression level of PepO and phosphorylated CovR in the emm1 wild-type A20 strain, its rocA mutant (ΔrocA), SPY362 (emm49), and its vector-control (Vec), and the rocA trans-complementary strain (ProcA). (B) Expression levels of PepO and phosphorylated CovR in the emm1 wild-type A20 strain, its rocA mutant (ΔrocA), the RocA-truncated mutant (rocAemm3), and the emm3-type isolates. PepO and phosphorylated CovR were detected by Western blotting and Phostag Western blotting, respectively, from a total protein extract. CovR~P denotes phosphorylated CovR; CovR denotes non-phosphorylated CovR. Lower panels of images show total protein as the internal loading control. An arrow indicates the PepO signal on SDS-PAGE.