TABLE 3.
Primers used in this studya
| Primer | Use | Sequence (5′–3′)b | Reference |
|---|---|---|---|
| PhasA-F | PCR | tcagatgaagttgtactccctgaa | This study |
| hasB-R-1 | PCR/sequencing | gctcaatcataccaccaact | This study |
| hasA-seq-F | Sequencing | atcgaggtccctgtctttca | This study |
| hasA-seq-R | Sequencing | cgttttgaagtgataaaagaactcc | This study |
| hasB-seq-F | Sequencing | ggaatggggaacacgtaaaa | This study |
| pepO-F | qPCR | tgcctttaaagagcgcacag | This study |
| pepO-R | qPCR | ctaccccacctagatcagcg | This study |
| Orf-1-F | Construction | gttaaaaggaggcgcctactTAgtggttattgttactatttttg | 38 |
| Orf-1-R | Construction | caaaaatagtaacaataaccacTAagtaggcgcctccttttaac | 38 |
| SIP-F-1 | Construction | gcgggatccggtcaatagccagatgcgata | 38 |
| SIP-R-1 | Construction | gcgggatcctcgtgatagggtccacaaca | 38 |
| gyrA-F-3 | qPCR | cgtcgtttgactggtttgg | 33 |
| gyrA-F-3 | qPCR | ggcgtgggttagcgtattta | 33 |
| PepO-BamHI-F | Construction | gcg ggatcc cgagacatcaatgtcgaaaca | This study |
| PepO-BamHI-R | Construction | gcg ggatcc gaatctcgtgcaatggttgat | This study |
| PepO-SacII-F | Construction | tcc ccgcgg taattaggtagttgataaacc | This study |
| PepO-SacII-R | Construction | tcc ccgcgg cattcgtatctccttatatca | This study |
| promoter-pepO-F | Construction | gcg ggatcc aacgccatttaggtgaccag | This study |
| promoter-pepO-R | Construction | gcg ggatcc tgcttttgcacgttttgaag | This study |
a
qPCR, quantitative PCR.
b
The underline indicates the restriction enzyme site.