Figure 3.

Autoradiograms of 20% denaturing PAGE, showing the cleavage kinetics of 5′-[³²P]-labelled RNA 4 in duplexes containing ASO 2 or B-ASO-2 (2a₁, 2b₁, 2c₁), (panel a) after treatment with RNase H (1 µL of 0.3 U/µL stock solution) at 0, 5, 15, 30, 60, and 90 min. Densitometric analyses of the kinetics of intact 5′-[³²P]-RNA 4 substrate were performed using three independent PAGE analysis experiments (panel b). Red arrows indicate products of the degraded 5′-[³²P]-RNA 4. Experimental conditions are described in the Materials and Methods section. Figure were created in BioRender (https://biorender.com/, accessed on 23 June 2022) and GraphPad Prism (https://www.graphpad.com/, accessed on 15 August 2022).